Human CD200R2 (CD200 Receptor 2) ELISA Kit | ELK6661

Human CD200R2 (CD200 Receptor 2) ELISA Kit | ELK6661 | Gentaur UK, US & Europe Distribution

Alternative Names: CD200R1L; CD200RLa; HuCD200R2; Cell surface glycoprotein CD200 receptor 2; CD200 cell surface glycoprotein receptor-like a; Cell surface glycoprotein OX2 receptor 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.34 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD200 Receptor 2 (CD200R2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CD200 Receptor 2 (CD200R2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD200 Receptor 2 (CD200R2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD200 Receptor 2 (CD200R2) in the samples is then determined by comparing the OD of the samples to the standard curve.