749

Human CD274/PD-L1 protein, His tag (Animal-Free) | PRP2021

(No reviews yet) Write a Review
SKU:
749-PRP2021
Availability:
Usually ships in 5 working days
NULL135.00 - NULL1,498.00

Description

Human CD274/PD-L1 protein, His tag (Animal-Free) | PRP2021 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines (Animal-Free)

Sequence: Amino acid sequence derived from human CD274 (NP_054862.1) (Met1-Thr239) was expressed with a 6 His tag at the C-terminus.

Activity: N/A

protein Lenght: The recombinant human CD274 consists of 227 amino acids and predicts a molecular mass of 26.8 KD. It migrates as an approximately 33-40 KD band in SDS-PAGE under reducing conditions due to glycosylation.

Preparation: HEK293 Cells

Purity: >98% as determined by SDS-PAGE

Formulation: Lyophilized from sterile PBS, pH 7.4.

Kit Component: N/A

Features & Benefits : Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Molecular Weight: 26.8 KD

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD274/PD-L1 protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human CD274/PD-L1 protein, His tag (Animal-Free) should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.24% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. Plays a critical role in induction and maintenance of immune tolerance to self . As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL-10) . The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity) . The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.

Alternative Names: Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1

Search Names: Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1

Tag: CD274/PD-L1

View AllClose