Human CD3E protein, Fc tag (Animal-Free) | PRP2029

Human CD3E protein, Fc tag (Animal-Free) | PRP2029 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines (Animal-Free)

Sequence: Amino acid sequence derived from human CD3E protein (P07766, Asp 23-Asp 126) was expressed with the Fc region of human IgG1 at the C-terminus.

Activity: N/A

protein Lenght: The recombinant human CD3E protein consists of 335 amino acids and predicts a molecular mass of 38 kD. The apparent molecular mass of the human CD3E is approximately 40-50 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Preparation: HEK 293 Cells

Purity: >95% as determined by SDS-PAGE

Formulation: 0.22 µm filtered protein solution is in PBS, pH 7.4.

Kit Component: N/A

Features & Benefits : Endotoxin: <0.1 EU per µg of the protein as determined by the LAL method.

Molecular Weight: 38 kDa

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized human CD3E protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human CD3E protein, Fc tag (Animal-Free) should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.108% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: CD3E is a one-way type I membrane glycoprotein on the surface of t cells. As a Part of the TCR-CD3 complex present on T-lymphocyte cell surface, CD3E plays an essential role in adaptive immune response. CD3E contains one Ig-like domain and one ITAM domain. CD3E together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor α/β and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition with a variety of intracellular signal transduction pathways. Polypeptides play an important role in T cell development. CD3E molecules play a crucial role in T cell development, and CD3e gene deficiency can lead to severe immune deficiency. The CD3E gene is also associated with a woman's susceptibility to type I diabetes. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2. The CD3 co-receptor complex is essential for signal transduction after specific binding of the T-cell receptor (TCR) .

Alternative Names: CD3e Molecule ;CD3e Antigen, Epsilon Polypeptide (TiT3 Complex) ;T-Cell Surface Glycoprotein CD3 Epsilon Chain;T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex) ;T3E;T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3 ;CD3e Antigen;CD3-Epsilon;IMD18 ;TCRE ;CD3E

Search Names: CD3e Molecule ;CD3e Antigen, Epsilon Polypeptide (TiT3 Complex) ;T-Cell Surface Glycoprotein CD3 Epsilon Chain;T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex) ;T3E;T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3 ;CD3e Antigen;CD3-Epsilon;IMD18 ;TCRE ;CD3E

Tag: CD3E