Human CD80/B7-1 protein, His tag (Animal-Free) | PRP2028

Human CD80/B7-1 protein, His tag (Animal-Free) | PRP2028 | Gentaur UK, US & Europe Distribution

Product Category: Cytology

Application: Cytokines

Product Type: Cytokines (Animal-Free)

Sequence: Amino acid sequence derived from human CD80 (P33681-1) (Met1-Asn242) was expressed with a 6 His tag at the C-terminus.

Activity: N/A

protein Lenght: The recombinant human CD80/B7-1 protein consists of 214 amino acids and predicts a molecular mass of 25.4 KD. It migrates as an approximately 40-60 KD band in SDS-PAGE under reducing conditions due to glycosylation.

Preparation: HEK293 Cells

Purity: >95% as determined by SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.

Kit Component: N/A

Features & Benefits : Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Molecular Weight: 25.4 KD

Usage Note: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD80/B7-1 protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

Storage Conditions: Lyophilized Human CD80/B7-1 protein, His tag (Animal-Free) should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.104% HSA or BSA) . Please prevent freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.

Background: B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses . Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response . Mature mouse B7-1 consists of a 209 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 22 aa transmembrane segment, and a 38 aa cytoplasmic domain . Within the ECD, mouse B7-1 shares 50% and 68% aa sequence identity with human and rat B7-1, respectively. Alternative splicing generates a short isoform that lacks the second Ig-like domain but retains the ability to bind CD28 . Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 . B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.

Alternative Names: CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80

Search Names: CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80

Tag: CD80/B7-1