Human CDKN3 (Cyclin Dependent Kinase Inhibitor 3) ELISA Kit | ELK3608

Human CDKN3 (Cyclin Dependent Kinase Inhibitor 3) ELISA Kit | ELK3608 | Gentaur UK, US & Europe Distribution

Alternative Names: CDI1; CIP2; KAP1; CDK2-Associated Dual Specificity Phosphatase; Kinase Associated Phosphatase; Cyclin-dependent kinase interactor 1; Cyclin-dependent kinase-interacting 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 3 (CDKN3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cyclin Dependent Kinase Inhibitor 3 (CDKN3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase Inhibitor 3 (CDKN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase Inhibitor 3 (CDKN3) in the samples is then determined by comparing the OD of the samples to the standard curve.