Human Cell surface glycoprotein CD200 receptor 2 (CD200R1L) ELISA Kit | KTE62427

Human Cell surface glycoprotein CD200 receptor 2 (CD200R1L) ELISA Kit | KTE62427 | Gentaur UK, US & Europe Distribution

Application: This Human Cell surface glycoprotein CD200 receptor 2 (CD200R1L) ELISA Kit employs a two-site sandwich ELISA to quantitate CD200R1L in samples. An antibody specific for CD200R1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCD200R1L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD200R1L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD200R1L bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Cell surface glycoprotein CD200 receptor 2 microplate
• Human Cell surface glycoprotein CD200 receptor 2 standard
• Human Cell surface glycoprotein CD200 receptor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Cell surface glycoprotein CD200 receptor 2 (CD200R1L) ELISA Kit has high sensitivity and excellent specificity for detection of Human CD200R1L. No significant cross-reactivity or interference between Human CD200R1L and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: CD200 delivers immunoregulatory signals following engagement of its receptor, CD200R. A family of CD200Rs (CD200R1-4) has been described. Spleen expresses cell surface CD200R1, while bone marrow shows predominantly expression of cell surface CD200R2/R3. Dendritic cell precursors (DCp) cultured with anti-CD200R2/3 develop the capacity to induce CD4 (+) CD25 (+) regulatory T cells (Treg) from peripheral lymphocytes. Foxp3 (+) CD4 (+) CD25 (+) Treg were derived from 60-hr thymocyte and splenocyte T cell cultures using both DC populations. Cloned C3H Treg (Foxp3 (+) ) suppressed both C3H anti-BL/6 reactivity in a fresh MLC and rejection of BL/6 skin allografts in C3H recipients; the converse was true for BL/6 Treg. CD200 triggering of bone-marrow DCs in the absence of CD200R1 engagement induces CD4 (+) CD25 (+) Treg, and these cloned antigen-specific Treg may have clinical utility.

Alternative Names: CD200R1L; CD200R2; CD200RLa; CD200 cell surface glycoprotein receptor 2; CD200 receptor 2

Search name: CD200R1L; CD200R2; CD200RLa; CD200 cell surface glycoprotein receptor 2; CD200 receptor 2

Tag: CD200R1L