Human CellExp™ 4-1BB / TNFRSF9, Human recombinant | 9227

4-1BB is also known as CD137, tumor necrosis factor receptor superfamily member 9 (TNFRSF9), induced by lymphocyte activation (ILA), is a co-stimulatory molecule of the tumor necrosis factor (TNF) receptor superfamily. CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. The best characterized activity of CD137 is its costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice. CD137 can enhance activation-induced T cell apoptosis when triggered by engagement of the TCR/CD3 complex. In addition, 4-1BB/4-1BBL co-stimulatory pathway has been shown to augment secondary CTL responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF. responses to several viruses, and meanwhile augment anti-tumor immunity. 4-1BB thus is a promising candidate for immunotherapy of human cancer. CD137 has been shown to interact with TRAF

9227 | Human CellExp 4-1BB / TNFRSF9 Human recombinant DataSheet

Biomolecule/Target: CD137

Synonyms: TNFRSF9,4-1BB,CD137,CDw137,ILA

Alternates names: Inhibin beta-1, FRP, FSH-releasing protein

Taglines: A transmembrane cytokine

NCBI Gene ID #: 3624

NCBI Gene Symbol: INHBA

Gene Source: Human

Accession #: P08476

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 92%

Assay #2: N/A

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: The activity is determined by the ability to induce cytoxicity of MPC-11 cells and is typically less than 2 ng/mL.

Results: < 2 ng/mL

Binding Capacity: Immobilized Human 4-1BB, His Tag at 100 ng/Ml (100 µl/well),can bind Human 4-1BB Ligand, Fc Tag with a linear of 0.1-2.5 ng/mL.

Unit Definition: N/A

Molecular Weight: 26.2 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water, which can then be further diluted to other aqueous solutions.

Amino acid sequence: MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS