Human CellExp™ Acid Sphingomyelinase, Human Recombinant | P1443

Sphingomyelin phosphodiesterase, which is encoded by the SMPD1 gene, is also known as acid sphingomyelinase or aSMase. There are two types of sphingomyelinases: ASM (acid), and NSM (neutral). ASM / aSMase can catalyze the hydrolysis of sphingomyelin to ceramide and phosphorylcholine with cofactor Zn2+. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation. Mutations in the SMPD1 gene cause Niemann–Pick disease types A and B due to deficiency in hydrolyzing sphingomyelin to ceramide. Activation of ASM can be achieved by the removal of its C terminal cysteine residue or C-terminal truncation. ’s recombinant human ASM was expressed from HEK293 cells without the last three C terminal residues, and is therefore constitutively active.

P1443 | Human CellExp™ Acid Sphingomyelinase, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Sphingomyelin phosphodiesterase, aSMase, acid sphingomyelinase, SMPD1, ASM, EC:3.1.4.12

Alternates names: Sphingomyelin phosphodiesterase, aSMase, acid sphingomyelinase, SMPD1, ASM, EC:3.1.4.12

Taglines: Catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine

NCBI Gene ID #: 6609

NCBI Gene Symbol: SMPD1

Gene Source: Human

Accession #: P17405

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: > 95%

Assay: SDS-PAGE.

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): The specific activity is >2 U/mg. Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC, freshly dissolved in DMSO) which can be detected at 420 nm.

Biological activity: The specific activity is >2 U/mg. Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC, freshly dissolved in DMSO) which can be detected at 420 nm.

Results:

Binding Capacity:

Unit Definition: One unit of aSMase/ASM is defined as the amount of enzyme that hydrolyzes 1.0 umol of substrate HNPPC per min under the assay conditions at 37 ºC.

Molecular Weight: 68 kDa (C-terminal 8×His tag)

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in distilled water. Do not vortex.

Amino acid sequence: His 62 - Pro 628

Handling: Centrifuge the vial prior to opening.

Usage: