Human CellExp™ APRIL / TNFSF13, Human Recombinant | P1372

APRIL (a proliferation-inducing ligand) is also known as Tumor necrosis factor ligand superfamily member 13, TALL-2, TRDL-1, CD256, TNFFSF 13, cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. APRIL is a cytokine of the tumor necrosis factor family associated mainly with hematologic malignancies. The closely related TNF family ligands B cell activation factor (BAFF) and a proliferation-inducing ligand (APRIL) serve in the generation and maintenance of mature B-lymphocytes. Both BAFF and APRIL assemble as homotrimers that bind and activate several receptors that they partially share. BAFF-APRIL heteromers of different stoichiometries have distinct receptor-binding properties and activities. In addition, expression of APRIL was regulated by miR-145 in GC cells.

P1372 | Human CellExp APRIL / TNFSF13 Human Recombinant DataSheet

Biomolecule/Target: TNFSF13

Synonyms: APRIL, TNFSF13, TALL-2, TRDL-1, CD256, TALL2, ZTNF2

Alternates names: GranzymeB, Granzyme-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, SECT, Cathepsin G-like 1, CTSGL1, CTLA-1, Fragmentin-2, Human lymphocyte protein, HLP, C11

Taglines: Plays a role in the regulation of tumor cell growth.

NCBI Gene ID #: 181186

NCBI Gene Symbol: Granzyme B

Gene Source: Human

Accession #: AAA75490

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA

Biological activity: Activity of GZMB was tested using the synthetic peptide, IETD-AFC, as a substrate. GZMB releases AFC and then the GZMB activity can be quantified by fluorescent detection of free AFC at Ex/Em=380/500 nm.

Results: 40 units/g

Binding Capacity: Immobilized Human BCMA, Mouse IgG2a Fc Tag at 5 g/mL (100 L/well) can bind Human APRIL, Fc Tag with a linear range of 0.6-10 ng/mL

Unit Definition: N/A

Molecular Weight: 32 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Use 50 µl sterile D.I. water to dissolve 5 µg protein to a final conc of 0.1 µg protein/µl.

Amino acid sequence: N/A