Human CellExp™ Carbonic Anhydrase IX / CA9, Human Recombinant | P1336

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes. CAs form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. One of the functions of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. The active site of most carbonic anhydrases contains a zinc ion. There are at least five distinct CA families (, , , and ). Carbonic anhydrase 9 (CA9 / CAIX), also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.

P1336 | Human CellExp Carbonic Anhydrase IX / CA9 Human Recombinant DataSheet

Biomolecule/Target: CA9

Synonyms: CAIX, CA9, CA-IX, G250, MN, P54/58N, pMW1

Alternates names: CAIX, CA9, CA-IX, G250, MN, P54/58N, pMW1

Taglines: A zinc metalloenzyme serving as a novel specific biomarker for a cervical neoplasia.

NCBI Gene ID #: 2921

NCBI Gene Symbol: GRO-

Gene Source: Human

Accession #: P19876

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 36 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in 20 mM MES and 100 mM NaCl, pH 6.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Amino acid sequence: N/A