Human CellExp™ CD19, Human recombinant | 9246

B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.

9246 | Human CellExp CD19 Human recombinant DataSheet

Biomolecule/Target: CD19

Synonyms: CD19, B4, CVID3, MGC12802

Alternates names: Trefoil Factor 2, Spasmolytic polypeptide (SP), Spasmolysin

Taglines: B-lymphocyte antigen CD19

NCBI Gene ID #: 7032

NCBI Gene Symbol: TFF2

Gene Source: Human

Accession #: Q03404

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: >95%

Assay #2: N/A

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human monocytes using a concentration range of 1.0-10.0 µg/ml.

Results: 1.0-10.0 µg/ml

Binding Capacity: Immobilized FMC63 mAb at 2g/mL(100L/well) can bind Human CD19 (20-291), His Tag with a linear range of 0.04-0.6 g/mL.

Unit Definition: N/A

Molecular Weight: 12.0 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 g/l. The solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A