Human CellExp™ CD23, human recombinant | 7435

Cluster of differentiation 23 (CD23), also known as Low affinity immunoglobulin epsilon Fc receptor (FCER2), C-type lectin domain family 4 member J (CLEC4J), Fc-epsilon-RII (FcRII), Immunoglobulin E-binding factor (IGEBF), is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. Unlike many of the antibody receptors, CD23 is a C-type lectin. It is found on mature B cells, activated macrophages, eosinophils, follicular dendritic cells, and platelets. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. CD23 is known to have role of transportation in antibody feedback regulation. Antigen that enters the blood stream is captured by antigen specific IgE antibodies. The IgE immune complexes that are formed bind to CD23 molecules on B cells, and are transported to the B cell follicles of the spleen. The antigen is then transferred from CD23+ B cells to CD11c+ antigen presenting cells. The CD11c+ cells in turn present the antigen to CD4+ T cells, which can lead to an enhanced antibody response. In flow cytometry, CD23 is helpful in the differentiation of chronic lymphocytic leukemia (CD23-positive) from mantle cell leukemia (CD23-negative).

7435 | Human CellExp CD23 human recombinant DataSheet

Biomolecule/Target: CD23

Synonyms: FCER2; FCER-2; CD23; CD-23; CD23A; CD-23A; CLEC4J; FCE2; FCE-2; IGEBF; FcRII; FcR; Fc epsilon RII

Alternates names: Trefoil factor 3 (Intestinal trefoil factor) (hITF) (Polypeptide P1.B) (hP1.B)

Taglines: Mediates transportation during antibody feedback regulation.

NCBI Gene ID #: 7033

NCBI Gene Symbol: TFF3

Gene Source: Human

Accession #: Q07654

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/ml.

Results: 1.0-10.0 ng/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 13.2 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Reconstitute in sterile 18 M-cm HO at not less than 100 µg/ml. Reconstituted TFF3 should be stored in working aliquots at -20°C.

Amino acid sequence: EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF