Human CellExp™ CD25/IL-2R alpha, C-His Tag, Human Recombinant | P1303

Interleukin-2 receptor subunit alpha (IL2RA) is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells. IL2RA binds IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covalently with the IL2 receptor beta and gamma chain, and subsequently initiates intracellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been considered as an activation marker. While both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.

P1303 | Human CellExp CD25/IL-2R alpha C-His Tag Human Recombinant DataSheet

Biomolecule/Target: IL-2R

Synonyms: IL2RA, Interleukin-2 receptor subunit alpha, p55, CD25, IL2-RA, TAC antigen,

Alternates names: INT-1, Proto-oncogene Int-1 homolog

Taglines: Extracellular Domain of IL-2R fused to 6x His tag at the C-terminus.

NCBI Gene ID #: 7471

NCBI Gene Symbol: WNT1

Gene Source: Human

Accession #: P04628

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.

Results: 1.5 - 2.5 ng/m

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 38.4 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 m filtered PBS (pH 7.4) with 5% trehalose.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 µg/µl. The solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A