Human CellExp™ CD27 / TNFRSF7, Fc Tag, Mouse Recombinant | P1406

CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.

P1406 | Human CellExp™ CD27 / TNFRSF7, Fc Tag, Mouse Recombinant DataSheet

Biomolecule/Target :

Synonyms: CD27, TNFRSF7, S152, T14, Tp55

Alternates names: CD27, TNFRSF7, S152, T14, Tp55

Taglines: Member of Tumor necrosis factor receptor superfamily, play a role in survival of activated T-cells and in apoptosis through association with SIVA1.

NCBI Gene ID #: 21940

NCBI Gene Symbol: Cd27

Gene Source: Murine

Accession #: P41272-1

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: >95%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level: Less than 0.1 EU per ug by the LAL method

Activity (Specifications/test method): Immobilized Mouse CD27, Mouse IgG2a Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Mouse CD70, His Tag, Avi Tag with a linear range of 1-16 ng/m. Immobilized Mouse CD27, Mouse IgG2a Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 1-16 ng/mL

Biological activity:

Results:

Binding Capacity: Immobilized Mouse CD27, Mouse IgG2a Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Mouse CD70, His Tag, Avi Tag with a linear range of 1-16 ng/m. Immobilized Mouse CD27, Mouse IgG2a Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 1-16 ng/mL

Unit Definition:

Molecular Weight: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Amino acid sequence:

Handling: Centrifuge the vial prior to opening.

Usage: