Human CellExp™ CD3 epsilon & CD3 delta Heterodimer | P1183

T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon (CD3E), CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

P1183 | Human CellExp CD3 epsilon & CD3 delta Heterodimer Human Recombinant DataSheet

Biomolecule/Target: CD3E, CD3D

Synonyms: CD3E & CD3D, CD3 delta & CD3 epsilon, CD3 delta / epsilon

Alternates names: CD3E & CD3D, CD3 delta & CD3 epsilon, CD3 delta / epsilon

Taglines: T cell receptor plays an important role in coupling antigen recognition

NCBI Gene ID #: 3458

NCBI Gene Symbol: IFNG

Gene Source: Human

Accession #: P01579

Recombinant: Yes

Source: Expi293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Human CD3 epsilon & CD3 delta Heterodimer Protein was immobilized at 0.5 µg/ml (100 µl/well). Dose response curve using Anti-hCD3E/CD3D Ab foralumab gave an EC of 21.69 ng/ml by ELISA. Human CD3 epsilon & CD3 delta Heterodimer Protein was Immobilized at 0.1 µg/ml (100 µl/Well). Dose response curve using Anti-hCD3E/CD3D Ab OKT3 gave an EC of 21 ng/ml by ELISA.

Results: N/A

Binding Capacity: Immobilized OKT3 MAb at 5 g/mL (100 L/well) can bind Human CD3E & CD3D, His Tag with a linear range of 0.625-5 g/mL

Unit Definition: N/A

Molecular Weight: 16.7 kDa and 13.5 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS pH 7.4. Generally 5-8% Mannitol or Trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Amino acid sequence: CD3E (Asp 23-Asp 126) and CD3D (Phe 22-Ala 105) (Fc tag at C-terminal)