Human CellExp™ CD33/SIGLEC-3 | P1616

CD33 is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This single-pass type I membrane protein contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like Vtype (immunoglobulin-like) domain. It is considered myeloid-specific, but it can also be found on some lymphoid cells. It binds to α2-6- and α2-3-sialylated glycans and sialylated ligands. It acts as an inhibitory receptor since, when it is crosslinked, it becomes phosphorylated on its cytoplasmic ITIM sequence that recruits SHP-1 and SHP-2 phosphatases and negatively regulates cell activation in both myeloid cell lines and activated NK cells. It induces apoptosis in acute myeloid leukemia and is a target for the treatment of leukemia.

P1616 | Human CellExp™ CD33/SIGLEC-3 DataSheet

Biomolecule/Target :

Synonyms: gp67; Siglec-3; CD33; Sialic acid binding Ig-like lectin 3; Myeloid cell surface antigen CD33

Alternates names: gp67; Siglec-3; CD33; Sialic acid binding Ig-like lectin 3; Myeloid cell surface antigen CD33

Taglines: Human recombinant with Fc & His Tag

NCBI Gene ID #: 945

NCBI Gene Symbol:

Gene Source: Human

Accession #: P20138; NP_001763.3

Recombinant: TRUE

Source: HEK293 cells

Purity by SDS-PAGE: ≥ 97% by SDSPAGE

Assay:

Purity:

Assay #2:

Endotoxin Level: < 0.1 EU/ug of the protein as determined by LAL method.

Activity (Specifications/test method):

Biological activity: The activity was measured by the binding ability in a functional ELISA. Immobilized Human CD33 at 2 ug/ml (100 ul/well) can bind Anti-Human CD33 Antibody in the linear range of 8-20 ng/ml.

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: The protein migrates as a band at 70 kDa (Fc and 6×His tag at the C-terminus).

Concentration:

Appearance: Lyophilized protein

Physical form description:

Reconstitution Instructions: Reconstitute to a concentration of 0.1-0.5 mg/ml in sterile distilled water.

Amino acid sequence: aa Asp18-His259 of human protein. The target protein is expressed with the sequence (Asp18-His259) of human CD33/Siglec-3 fused with an Fc and 6×His tag at the C terminus.

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans