Human CellExp™ CD73 / NT5E, Human recombinant | P1379

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

P1379 | Human CellExp CD73 / NT5E Human recombinant DataSheet

Biomolecule/Target: CD73 / NT5E

Synonyms: CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE

Alternates names: CD73, NT5E, 5'-Nucleotidase, 5'-NT, NT5, NTE

Taglines: Enzyme that convert AMP to adenosine

NCBI Gene ID #: 15978

NCBI Gene Symbol: IFNG

Gene Source: Human

Accession #: P01580

Recombinant: TRUE

Source: HEK293

Purity by SDS-PAGEs: 95%

Assay: N/A

Purity: N/A

Assay #2: N/A

Endotoxin Level: Less than 1.0 EU per g by the LAL method.

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 59.9 kDa. The protein migrates as 30 kDa, 40 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. It is recommended to store at -20°C.

Amino acid sequence: AA Trp 27 - Ser 549