Human CellExp™ Complement C5, Cynomolgus Recombinant | P1414

Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

P1414 | Human CellExp™ Complement C5, Cynomolgus Recombinant DataSheet

Biomolecule/Target :

Synonyms: Complement C5,C5,CPAMD4

Alternates names: Complement C5,C5,CPAMD4

Taglines: A potent chemokine which stimulates the locomotion of polymorphonuclear leukocyte

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Cynomolgus

Accession #: XP_005580972.1

Recombinant: False

Source: human 293 cells (HEK293)

Purity by SDS-PAGE: >95%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level: Less than 1.0 EU per ug by the LAL method

Activity (Specifications/test method): Immobilized Cynomolgus Complement C5, His Tag at 2 ug/ml (100 ul/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-6 ng/ml.

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 73.3 kDa (β chain) and 114.9 kDa (α chain). The protein migrates as 70 kDa (β chain) and 116 kDa (α chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration:

Appearance: Sterile Filtered colorless solution

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Amino acid sequence: AA Gln 19 - Cys 1676

Handling: Centrifuge the vial prior to opening.

Usage: