Human CellExp™ Contactin-5 (His Tag), Human Recombinant | P1748

Human CellExp™ Contactin-5 (His Tag), Human Recombinant | P1748

Description: Contactin-5 (CNTN5) is a member of the immunoglobulin superfamily and functions to mediate cell surface interactions during nervous system development. Contactin-5 may play a role in the formation of axon connections in the developign nervous system. Contactin-5 has been implicated in neurite outgrowth-promoting activity in cerebral cortical neurons, as well as neuronal activity in the auditory system.

Biomolecule/Target:

Synonyms: CNTN5; HNB-2; NB-2; Neural recognition molecule NB-2; Neural Adhesion Molecule; HNB-2s

Alternates names:

Taglines: Cell adhesion molecule that may play a role in neural development

NCBI Gene ID #: 53942

NCBI Gene Symbol: Human

Accession #: O94779

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: > 90% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Activity (Specifications/test method): Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10^4 cells/well are added to CNTN5 coated plates (12.5μg/ml and 100 μl/well), approximately 30%-50% will adhere specifically after 60 minutes at 37 °C

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: ~130-140 kDa (observed due to glycosylation)

Concentration:

Appearance: Lyophilized protein

Physical form description: Lyophilized powder

Reconstitution Instructions:

Amino acid sequence: Glu 19 to Gln 1059 with C-terminal polyhistidine tag

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans