Human CellExp™ DKK-1, Human Recombinant | 7132

DKK-1 is a member of the DKK protein family which also includes DKK-2, DKK-3 and DKK-4. DKK-1 was originally identified as a Xenopus head forming molecule that behaves as an antagonist for Wnt signaling. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/ catenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/ catenin signaling cascade. It has been suggested that by inhibiting Wnt/ catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Recombinant human DKK-1 expressed in human 293 cells is a 35-40 kDa glycoprotein containing 235 amino-acid residues.

7132 | Human CellExp DKK-1 Human Recombinant DataSheet

Biomolecule/Target: DKK-1

Synonyms: Dickkopf-related protein-1, Dickkopf-1, SK

Alternates names: Melanoma antigen recognized by T-cells 1, Antigen LB39-AA, Antigen SK29-AA, MART-1, Melan-A

Taglines: Plays an important regulatory role in the Wnt /-catenin signaling pathway

NCBI Gene ID #: 77836

NCBI Gene Symbol: Melan-A

Gene Source: Human

Accession #: Q2TA50

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: N/A

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200 ng/ml.

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.6 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 1X PBS, pH 7.5.

Reconstitution Instructions: N/A

Amino acid sequence: N/A