Human CellExp™ GHR /Growth Hormone Receptor, human recombinant | 7477

Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

7477 | Human CellExp GHR /Growth Hormone Receptor human recombinant DataSheet

Biomolecule/Target: GHR /Growth Hormone Receptor

Synonyms: GHR, GHBP, GH receptor

Alternates names: GHR, GHBP, GH receptor

Taglines: A receptor for pituitary gland growth hormone involved in regulating postnatal body growth

NCBI Gene ID #: 3481

NCBI Gene Symbol: IGF2

Gene Source: Human

Accession #: P01344

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: The bioactivity is measured by its binding ability to human GH in a SPR assay. Covalently immobilized Recombinant Human GHR Protein (Cat# GHR-H5222) can bind human growth hormone protein with affinity constant of 1.28 ±0.16 nM range.

Results: The bioactivity is measured by its binding ability to human GH in a SPR assay. Covalently immobilized Recombinant Human GHR Protein (Cat# GHR-H5222) can bind human growth hormone protein with affinity constant of 1.28 ±0.16 nM range.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 28.5 kDa. The predicted N-terminus is Ala 27. DTT-reduced Protein migrates as 40-50 kDa in SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Amino acid sequence: N/A