Human CellExp™ Granzyme B, human recombinant | 7233

Granzyme B (GZMB) also known as Cathepsin G-like 1 (CTSGL1), Cytotoxic T-lymphocyte proteinase 2 (Lymphocyte protease), Fragmentin-2, Granzyme-2, Human lymphocyte protein (HLP), SECT, CGL1, CSPB, CTLA1, GRB, which belongs to the peptidase S1 family. Granzyme subfamily GZMB / CTSGL1 contains 1 peptidase S1 domain. The catalytic activity of Granzyme B is “Preferential cleavage: -Asp-|-Xaa- >> -Asn-|-Xaa- > -Met-|-Xaa-, -Ser-|-Xaa-“, and is inactivated by the serine protease inhibitor diisopropyl fluorophosphates. GZMB is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. GZMB seems to be linked to activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution and cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

7233 | Human CellExp Granzyme B human recombinant DataSheet

Biomolecule/Target: Granzyme B

Synonyms: GZMB, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT, Fragmentin-2, Granzyme B, Granzyme-2, GRB

Alternates names: GZMB, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, HLP, SECT, Fragmentin-2, Granzyme B, Granzyme-2, GRB

Taglines: Involved in target cell lysis in cell-mediated immune responses

NCBI Gene ID #: 3481

NCBI Gene Symbol: IGF2

Gene Source: Human

Accession #: P01344

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: The protein is fused with 6×His tag at the C-terminus, has a calculated MW of 26.4 kDa. The predicted N-terminus is Thr 21. DTT-reduced Protein migrates as 35 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 m filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Amino acid sequence: N/A