Human CellExp™ Gremlin / GREM1, Human recombinant | P1124

Gremlin is also known as Cysteine knot superfamily 1, BMP antagonist 1 (CKTSF1B1), DAN domain family member 2 (DAND2), Down-regulated in Mos-transformed cells protein (DRM), Increased in high glucose protein 2 (IHG-2), Cell proliferation-inducing gene 2 protein (PIG2) or Gremlin-1 (GREM1), which is highly expressed in small intestine, fetal brain and colon. Gremlin / GREM-1 interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner. Gremlin may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Gremlin down-regulates the BMP4 signaling in a dose-dependent manner and acts as inhibitor of monocyte chemotaxis.

P1124 | Human CellExp Gremlin / GREM1 Human recombinant DataSheet

Biomolecule/Target: Grem1

Synonyms: GREM1, CKTSF1B1, DAND2, DRM, PIG2

Alternates names: Chorionic Somatomammotropin Hormone 1, CSH1, Choriomammotropin, Lactogen, CSH2, PL, CSA, CSMT, FLJ75407

Taglines: A TGF- antagonist

NCBI Gene ID #: 1442

NCBI Gene Symbol: CSH1

Gene Source: Human

Accession #: P01243

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): Measured by its binding ability in a functional ELISA.

Biological activity: Test in process

Results: In progress

Binding Capacity: Immobilized Human BMP-2, at 2 g/mL (100 L/well) can bind Human Gremlin, His Tag with a linear range of 0.8-81.9 ng/mL.

Unit Definition: N/A

Molecular Weight: 22.3 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A