Human CellExp™ IFN-gamma, Human Recombinant | 7271

Interferon-gamma (IFN-/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN- belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFN gamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.

7271 | Human CellExp IFN-gamma human recombinant DataSheet

Biomolecule/Target: IFN-gamma

Synonyms: IFNG, IFG, IFI

Alternates names: IFNG, IFG, IFI

Taglines: A central regulator of the immune response and signals

NCBI Gene ID #: 16175

NCBI Gene Symbol: IL1A

Gene Source: Human

Accession #: Q3U0Y6

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU/g by LAL method

Activity (Specifications/test method): Determined by a cytotoxicity assay using HT-29 cells. ED50 <0.05ng/ml, corresponding to a specific activity of >2X10^7 Unit/mg.

Biological activity: Measured by its binding ability in functional ELISA. Immobilized IFN-gamma at 5 µg/mL (100 µL/well) can bind Human IFN-gamma R1 with a linear range of 1-10 ng/mL.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: Calculated MW of 16.8 kDa with no tag. The predicted N-terminus is Gln 24. DTT-reduced protein migrates at 19 to 25 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.2 m filtered solution of PBS, pH 7.4, with 10% trehalose

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 200 µg/ml. Do not vortex. Aliquot and store at -20 °C.

Amino acid sequence: N/A