Human CellExp™ IL-1α, Human Recombinant | P1717

Interleukin-1 (IL-1) is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release. IL-1 also induces B-cell maturation and proliferation, and modulates fibroblast growth factor activity. IL-1 proteins have been identified as endogenous pyrogens, and are involved in the inflammatory response.

P1717 | Human CellExp™ IL-1α, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Interleukin-1 alpha; Hematopoietin-1; IL1; IL-1A; IL1F1; IL1-ALPHA; IL-1 alpha

Alternates names:

Taglines: Critical mediator of inflammatory response

NCBI Gene ID #: 3552

NCBI Gene Symbol:

Gene Source: Human

Accession #: P01583

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: ≥ 95% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level: < 1 EU per 1 ug of protein as determined by LAL method

Activity (Specifications/test method):

Biological activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells (ED50 typically 0.2-1 ng/mL) and by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells (ED50 typically 0.07-0.35 ug/mL)

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 18 kDa calculated; 22 kDa observed

Concentration:

Appearance: Liquid protein

Physical form description: Lyophilized powder

Reconstitution Instructions:

Amino acid sequence: Ser 113 to Ala 271 of human IL-1α

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans