Human CellExp™ IL-2 R alpha / CD25, Mouse Recombinant | P1464

Interleukin-2 receptor subunit alpha (IL2RA) is also known as IL-2R subunit alpha, IL-2-RA, IL2-RA, TAC antigen, p55, CD antigen CD25, is a type I transmembrane glycoprotein. IL2RA is expressed on activated T cells and regulatory T cells, and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covelently with the IL2 receptor beta and gamma chain, and subsequently initiates the intacellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling.

P1464 | Human CellExp™ IL-2 R alpha / CD25, Mouse Recombinant DataSheet

Biomolecule/Target :

Synonyms: IL2RA, CD25, p55, IL2-RA, IL-2-RA

Alternates names: IL2RA, CD25, p55, IL2-RA, IL-2-RA

Taglines: Extracellular Domain of IL-2R fused to 6x His tag at the C-terminus.

NCBI Gene ID #: 16184

NCBI Gene Symbol: Il2ra

Gene Source: Mouse

Accession #: P01590

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: >90%

Assay: SDS-PAGE

Purity:

Assay #2:

Endotoxin Level: Less than 1.0 EU per ug by the LAL method.

Activity (Specifications/test method):

Biological activity: Immobilized Biotinylated Human IL-2, Fc Tag, A at 5 ug/mL (100 uL/well) on streptavidin precoated (0.5 ug/well) plate, can bind Mouse IL-2 R alpha with a linear range of 5-40 ng/mL. Immobilized Mouse IL-2 R alpha at 5 ug/mL (100 uL/well) can bind Biotinylated Human IL-2 with a linear range of 0.6-10 ng/mL

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Amino acid sequence: Glu 22 - Lys 236

Handling: Centrifuge the vial prior to opening.

Usage: