Human CellExp™ IL-22, human recombinant | 7272

Interleukin-22 (IL22) is also known as cytokine Zcyto18, IL-10-related T-cell-derived-inducible factor (IL-TIF), which is belongs to the IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein IL-22BP. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10.

7272 | Human CellExp IL-22 human recombinant DataSheet

Biomolecule/Target: IL-22

Synonyms: IL-22, IL-TIF, ZCYTO18

Alternates names: IL-22, IL-TIF, ZCYTO18

Taglines: Initiates innate immune responses against bacterial pathogens

NCBI Gene ID #: 16176

NCBI Gene Symbol: IL1B

Gene Source: Human

Accession #: P10749

Recombinant: Yes

Source: HEK 293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g) by LAL method

Activity (Specifications/test method): Measured by its ability to induce IL-10 secretion in COLO 205 (human colon carcinoma cells). The ED50 for this effect is 0.5 - 1.0 ng/ml

Biological activity: Measured by its ability to activate STAT following receptor ligand interaction.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: Calculated MW of 17.2 kDa with no tag. The predicted N-terminus is Ala 34.

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized after extensive dialysis against PBS.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Amino acid sequence: N/A