Human CellExp™ Influenza A virus / Neuraminidase (NA) | 7508

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell.

7508 | Human CellExp Influenza A virus / Neuraminidase NA DataSheet

Biomolecule/Target: Influenza A virus / Neuraminidase (NA)

Synonyms: NA, Neuraminidase

Alternates names: NA, Neuraminidase

Taglines: 92% pure, active, human cell expressed Influenza A virus / Neuraminidase (NA) - a host cell receptor-destroying enzyme/glycoprotein found at the surface of influenza virus

NCBI Gene ID #: 16176

NCBI Gene Symbol: IL1B

Gene Source: Influenza A Virus

Accession #: P10749

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)--D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)--D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C

Results: Measured by its ability to cleave a fluorogenic substrate, 2'-(4-Methylumbelliferyl)--D-N-acetylneuraminic acid. One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)--D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme required to cleave 1 nmole of 2'-(4-Methylumbelliferyl)--D-N-acetylneuraminic acid per minute at pH 7.5 at 37°C

Molecular Weight: Influenza A virus (A/Thailand/1(KAN-1)/2004 (H5N1)) Neuraminidase (NA) is fused with a polyhistidine tag at the N-terminus, and has a calculated MW of 46.1 kDa. The predicted N-terminus is His 36. DTT-reduced Protein migrates as 48 kDa in SDS-PAGE

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Amino acid sequence: N/A