Human CellExp™ Kallikrein-3, human recombinant | 7415

Kallikrein-3 (KLK3) is also known as Prostate-specific antigen (PSA), APS, Gamma-seminoprotein (Seminin), P-30 antigen, SemenoGelase, is a glycoprotein enzyme, is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. The preferential cleavage site of KLK3 is “-Tyr-|-Xaa-“, the catalytic activity is inhibited by SERPINA5 and is strongly inhibited by Zn2+. KLK3 hydrolyzes semenoGelin-1 thus leading to the liquefaction of the seminal coagulum.

7415 | Human CellExp Kallikrein-3 human recombinant DataSheet

Biomolecule/Target: Kallikrein-3

Synonyms: PSA, KLK3, KLKB1, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, SemenoGelase

Alternates names: PSA, KLK3, KLKB1, Kallikrein-3, APS, KLK2A1, PSA, Hk3, Seminin, SemenoGelase

Taglines: Has important applications in the diagnosis of prostate cancer.

NCBI Gene ID #: UniGene:Rn.9869

NCBI Gene Symbol: IL1B

Gene Source: Human

Accession #: Q63264

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl – Arg – Pro – Tyr – p – Nitroanilide (Suc-RPY-pNA). The specific activity is >60 pmoles / min / g.

Results: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl – Arg – Pro – Tyr – p – Nitroanilide (Suc-RPY-pNA). The specific activity is >60 pmoles / min / g.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 27.7 kDa. The predicted N-terminus is Ala 18. DTT-reduced Protein migrates as 36 kDa due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Amino acid sequence: N/A