Human CellExp™ KLK-8 / Kallikrein-8, human recombinant | 7511

Kallikrein-8 (KLK8) is also known as Neuropsin (NP or NRPN), Ovasin, Serine protease 19 (PRSS19), Tumor-associated differentially expressed gene 14 protein (TADG-14), which belongs to the peptidase S1 family and Kallikrein subfamily. KLK8 contains 1 peptidase S1 domain. KLK8 is pH dependence protein and the optimum pH is 8.5, and the protein is active from pH 7-10. KLK8 is expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer. KLK8 cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys, and the catalytic activity of KLK8 is inhibited by a range of serine protease inhibitors including antipain, aprotinin, leupeptin, benzamidine and soybean trypsin inhibitor.

7511 | Human CellExp KLK-8 / Kallikrein-8 human recombinant DataSheet

Biomolecule/Target: KLK-8 / Kallikrein-8

Synonyms: KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8

Alternates names: KLK8, Kallikrein-8, Neuropsin, Ovasin, NRPN, PRSS19, TADG14, NP, hK8

Taglines: 95% pure, active, human cell expressed, human recombinant KLK-8 / Kallikrein-8 - serves as a marker of ovarian cancer.

NCBI Gene ID #: 3553

NCBI Gene Symbol: IL2

Gene Source: Human

Accession #: P60568

Recombinant: Yes

Source: HEK293 cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >500 pmoles / min / g.

Results: >500 pmoles / min / g

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 25.8 kDa. The predicted N-terminus is Gln 29. DTT-reduced Protein migrates as 38 kDa in SDS-PAGE due to glycosylation.

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in PBS, pH 7.4. Do not vortex.

Amino acid sequence: Gln 29 – Gly 260