Human CellExp™ LEFTY-I, Human Recombinant | 4873

’s LEFTY-1 is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 38 kDa. This cytokine is produced in a human cell expression system with serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation, contributing to stability in cell growth media. Lefty proteins function as an antagonist of the Nodal Signaling pathway. Proper functioning of Lefty is crucial to the proper development of the heart, lungs, spleen, and liver.

4873 | Human CellExp LEFTY-I Human Recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant LEFTY-I

Alternates names: Angiostatin K1-4, Angiostatin Kringles 1-4, plasminogen

Taglines: Plays a role in left-right asymmetry determination of organ systems during development

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Human

Accession #: P00747

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: The activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. The specific activity of anti-migration of endothelial cells in vitro is 0.55×10 Units/mg.

Results: 0.55×10 Units/mg.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 30.0 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized in 10 mM Tris-HCl, pH 7.4, 50 mM NaCl, 0.5% CHAPS.

Reconstitution Instructions: Reconstitute in sterile distilled HO or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Amino acid sequence: N/A