Human CellExp™ Nectin-4, Mouse Recombinant | P1477

Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.

P1477 | Human CellExp™ Nectin-4, Mouse Recombinant DataSheet

Biomolecule/Target :

Synonyms: NECTIN4, LNIR, PRR4, PVRL4

Alternates names: NECTIN4, LNIR, PRR4, PVRL4

Taglines: Transmembrane glycoprotein that involved in cell adhesion through trans-homophilic and -heterophilic interactions

NCBI Gene ID #: 71740

NCBI Gene Symbol: NECTIN 4

Gene Source: Mouse

Accession #: Q8R007-1

Recombinant: False

Source: HEK 293 cells

Purity by SDS-PAGE: >95%

Assay:

Purity:

Assay #2:

Endotoxin Level: Less than 1.0 EU per ug by the LAL method.

Activity (Specifications/test method): Immobilized Mouse Nectin-4, His Tag at 5 ug/mL (100 uL/well) can bind Human PVRL1 / NECTIN1 Protein, Fc Tag with a linear range of 0.039-1.25 ug/mL

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 36.2 kDa. The protein migrates as 38-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 100 ug/ml. Do not vortex. It is recommended to store at -20°C.

Amino acid sequence: Gly 31 - Ser 347

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans