Human CellExp™ OX40 Ligand / TNFSF4, Human recombinant | 9244

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

9244 | Human CellExp OX40 Ligand / TNFSF4 Human recombinant DataSheet

Biomolecule/Target: TNFRSF4

Synonyms: OX40L,TNFSF4,CD252

Alternates names: Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Taglines: A costimulatory molecule implicated in long-term T-cell immunity, also cts as a receptor for human herpesvirus 6B/HHV-6B

NCBI Gene ID #: 7422

NCBI Gene Symbol: VEGFA

Gene Source: Human

Accession #: P15692

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: >95%

Assay #2: N/A

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): N/A

Biological activity: Determined by the dose-dependent stimulation of the proliferation of human mbilical vein endothelial cells (HUVEC) using a concentration range of 0.1-0.5 ng/ml.

Results: 0.1-0.5 ng/ml

Binding Capacity: Immobilized HumanOX40, His Tag at 2 g/ml (100 l/well) can bind Human OX40 Ligand, Fc Tag with a linear range of 2-15 ng/ml.

Unit Definition: N/A

Molecular Weight: 28.4 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A