Human CellExp™ PDGFRb/CD140b, human recombinant | 7493

Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB), also called J03278, M21616, CD140B, JTK12, PDGF-R-beta and PDGFR, is receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity. Is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor (PDGF) family. The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (A, B, C and D). The receptors PDGFRA and PDGFRB are related in sequence and both are members of the class III subtype of receptor tyrosine kinases (RTKs). Other class III RTKs are CSF1R, KIT and FLT3. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the and receptors is independently regulated in various cell types. Recombinant soluble PDGFRB binds PDGF with high affinity and is potent PDGF antagonist. The ligands form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in their monomeric forms. The PDGFs bind to the protein tyrosine kinase receptors PDGF receptor- and -. These two receptor isoforms dimerize upon binding the PDGF dimer, leading to three possible receptor combinations, namely -, - and -. PDGF-CC specifically interacts with PDGFR- and -, but not with –, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR- with high affinity and to PDGFR- to a much lower extent and is regarded as PDGFR- specific. PDGF-AA binds only to PDGFR-, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity.

7493 | Human CellExp PDGFRb/CD140b human recombinant DataSheet

Biomolecule/Target: PDGFRb/CD140b

Synonyms: PDGFRB, PDGFR-B, CD140B, CD-140B, JTK12, JTK-12, PDGF-R-beta, PDGFR, PDGFR1, PDGFR-1, PDGFR-beta, PDGFR-, PDGF-R-

Alternates names: Tumor necrosis factor ligand superfamily member 15, TNFSF-15, TNFSF15, TNF ligand-related molecule 1, VEGI, TL-1, TL1, TL1A, VEGI192A, VEGI-192, MGC129934, MGC129935, Vascular endothelial cell growth inhibitor.

Taglines: Binds specifically to PDGFB and has a tyrosine-protein kinase activity

NCBI Gene ID #: 41019336

NCBI Gene Symbol: TNFSF15

Gene Source: Human

Accession #: AAR98573

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent inhibition of the proliferation of HUVEC is <5 ug/ml.

Results: <5 ug/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 21.8 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1–1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A