Human CellExp™ Recombinant Ebolavirus BDBV Envelope Glycoprotein 1 (GP1) | P1061

EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

P1061 | Human CellExp Recombinant Ebolavirus BDBV Envelope Glycoprotein 1 GP1 DataSheet

Biomolecule/Target: GP

Synonyms: GP1, GP, Envelope glycoprotein, GP2 (subtype Bundibugyo,strain Uganda 2007)

Alternates names: Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase.

Taglines: N/A

NCBI Gene ID #: 4282

NCBI Gene Symbol: MIF

Gene Source: Human

Accession #: P14174

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): N/A

Biological activity: Human MIF biological activity was measured in an agarose micro droplet assay using human U937 cells as targets. The optimal range of activity found for human MIF in this assay was 0.5-1µg/ml.

Results: 0.5-1µg/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 12.5 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in sterile dHO to a concentration of 0.1 -1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A