Human CellExp™ Renin, Human Recombinant | 6300

Renin is a highly specific endopeptidase with the known function of generating angiotensin I from angiotensinogen in the plasma. This enzymatic reaction with the particular substrate angiotensinogen initiates a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Prorenin is a glycosylated aspartic protease that consists of 2 homologous lobes and is the precursor of renin. Prorenin exhibits much lower level of enzymatic activity relative to activated renin. ’s active human renin is produced from the pro-form prorenin by removing the 43 amino acid N-terminal pro-segment with trypsin

6300 | Human CellExp Renin Human Recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Angiotensinogenase, REN.

Alternates names: Interleukin 31, IL31

Taglines: An aspartyl protease specific for angiotensinogen.

NCBI Gene ID #: 76399

NCBI Gene Symbol: IL31

Gene Source: Mouse

Accession #: Q6EAL8

Recombinant: True

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: A standard activity has not yet been determined.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of active human Renin is defined as the amount of enzyme that generates 1.0 µmol of substrate per min under the assay conditions at 37 ºC.

Molecular Weight: 15.7 kDa

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile HO at a concentration of 0.1mg/ml. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage. Reconstituted IL-31 should be stored in working aliquots at -20°C.

Amino acid sequence: MTCSLSFGAP ISKEDLRTTI DLLKQESQDL YNNYSIKQAS GMSADESIQL PCFSLDREAL TNISVIIAHL EKVKVLSENT VDTSWVIRWL TNISCFNPLN LNISVPGNTD ESYDCKVFVL TVLKQFSNCM AELQAKDNTT C