Human CellExp™ Sclerostin, human recombinant | P1145

Sclerostin (SOST) is also known as Sclerosteosis, VBCH, is a secreted glycoprotein with a signal peptide for secretion and a C-terminal cysteine knot-like (CTCK) domain and belongs to the Cerberus/DAN family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. More recently Sclerostin has been identified as binding to LRP5/6 receptors and inhibiting the Wnt signalling pathway. Wnt pathway inhibition under these circumstances is antagonistic to bone formation (meaning Sclerostin antagonizes bone formation). It has been shown that SOST binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. Sclerostin production by osteocytes is inhibited by parathyroid hormone, mechanical loading and cytokines including oncostatin M, cardiotrophin-1 and leukemia inhibitory factor. Sclerostin production is increased by calcitonin. Thus, osteoblast activity is self regulated by a negative feedback system. Mutations of Sclerostin is associated with the syndrome Sclerosteosis, and reduced sclerostin expression results in a milder form of the disorder called van Buchem disease.

P1145 | Human CellExp Sclerostin human recombinant DataSheet

Biomolecule/Target: SOST

Synonyms: SOST, VBCH

Alternates names: Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46Human Ghrelin (Ser3 modified), Ghrelin, h-Ghrelin, rh-Ghrelin, recombinant human Ghrelin, Ghrelin (Ser3 modified), recombinant Ghrelin, Ghrelin

Taglines: Negative regulator of bone growth

NCBI Gene ID #: 51738

NCBI Gene Symbol: GHRL

Gene Source: Human

Accession #: Q9UBU3

Recombinant: Yes

Source: N/A

Purity by SDS-PAGEs: N/A

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): Biological activity of ghrelin has not been established. The peptide can be used in ELISA, as a positive control in Western blot analysis, or as a blocking peptide to block antibody activity.

Biological activity:

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 3.374 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution Instructions: Reconstitute in PBS containing at least 0.1% BSA to a concentration of 1.0 mg/ml.

Amino acid sequence: GS(X)FLSPEHQRVQQRKESKKPPAKLQPR X = serine modified with n-octanoic acid