Human CellExp™ SERPIN F1, human recombinant | 7418

Serpin F1 (SERPINF1) is also known as Pigment epithelium-derived factor (PEDF), Cell proliferation-inducing gene 35 protein (PIG35). Serpin F1 belongs to the serpin family. Serpin F1 is expressed in quiescent cells. PEDF has a variety of functions including antiangiogenic, antitumorigenic, and neurotrophic properties. Endothelial cell migration is inhibited by SERPINF1/ PEDF. PEDF / SERPINF1 suppresses retinal neovascularization and endothelial cell proliferation. PEDF is also responsible for apoptosis of endothelial cells either through the p38 MAPK pathway or through the FAS/FASL pathway. PEDF also displays neurotrophic functions.

7418 | Human CellExp SERPIN F1 human recombinant DataSheet

Biomolecule/Target: SERPIN F1

Synonyms: SERPINF1, Serpin F1, PEDF, PIG35, EPC-1

Alternates names: Human PARP, PARP, h-PARP, rh-PARP, recombinant human PARP, PARPrecombinant PARP, PARP

Taglines: Has a variety of prpperties including antiangiogenic, antitumorigenic, and neurotrophic properties

NCBI Gene ID #: 142

NCBI Gene Symbol: PARP-1

Gene Source: Human

Accession #: P09874

Recombinant: Yes

Source: Baculovirus

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): N/A

Biological activity: 1000 U/vial, specific activity = 20000 U/mg PARP1. 1U=10 fmol ADP-ribose incorporated into 5 g immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 116.0 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Spin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 L dHO. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate. Upon reconstitution with 100 L dH20, the final concentrations are as follows: 0.5 mg/mL PARP1 enzyme, 20 mM Tris, pH 8, 0.3 M NaCl, 0.1 mM EDTA, 1 mM DTT, plus lyophilization stabilizers.

Amino acid sequence: N/A