Human CellExp™ Siglec-2, Fc Tag, Human Recombinant | P1487

Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8. Siglec-8 was first identified by CD33 homology screening of ESTs from a cDNA library generated from a patient diagnosed with idiopathic hypereosinophilic syndrome and was originally termed SAF-2 (sialoadhesin family 2). At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Two splice variants of Siglec-8 exist. The initially characterized form contains 431 amino acid residues in total, subsequently, a longer form of Siglec-8, initially termed Siglec-8L. Both forms of Siglec-8 are found in eosinophils and contain a V-set domain with lectin activity and two C2-type Ig repeat domains in the extracellular region.

P1487 | Human CellExp™ Siglec-2, Fc Tag, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Siglec-8, SAF-2, SIGLEC8, SAF2

Alternates names: Siglec-8, SAF-2, SIGLEC8, SAF2

Taglines: Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells

NCBI Gene ID #: 27181

NCBI Gene Symbol: SIGLEC8

Gene Source: Human

Accession #: Q9NYZ4-1

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: >95%

Assay:

Purity:

Assay #2:

Endotoxin Level: Less than 1.0 EU per ug by the LAL method.

Activity (Specifications/test method):

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 64.3 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Concentration:

Appearance: Lyophilized powder

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in sterile deionized water to the desired protein concentration.

Amino acid sequence: AA Met 17 - Ala 363

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans