Human CellExp™ SPAM1, human recombinant | 7247

Hyaluronidase PH-20 also known as Sperm adhesion molecule 1 (SPAM1) and Sperm surface protein PH-20, which belongs to the glycosyl hydrolase 56 family, SPAM1 / PH-20 is expressed in testis. SPAM-1 / PH20 random hydrolysis of (1->4)-linkages between N – acetyl – beta – D – glucosamine and D-glucuronate residues in hyaluronate. SPAM-1 / PH20 involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. SPAM1 aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

7247 | Human CellExp SPAM1 human recombinant DataSheet

Biomolecule/Target: SPAM1

Synonyms: SPAM1, PH-20, HYAL3, HYA1, HYAL1, HYAL5, SPAG15

Alternates names: C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor (C5a-R), (C5aR,) (CD antigen CD88), C5AR1, C5AR, C5R1

Taglines: Involved in sperm-egg adhesion

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source:

Accession #:

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): N/A

Biological activity: Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED = 1.36 nmolar.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: (74 amino acids)

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized from 0.22 m filtered solution in 50 mM Tris, 100 mM NaCl, pH 7.0-7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold HO containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C for future use. Frozen/thaw will result in some loss of activity.

Amino acid sequence: N/A