Human CellExp™ Thrombomodulin, Human Recombinant | 7215

Thrombomodulin (TM, CD141, and THBD) is an endothelial cell expressed transmembrane glycoprotein that can form a complex with the coagulation factor, Thrombin. The Thrombomodulin/Thrombin complex converts protein C to its activated form, protein Ca, which in turn proteolytically cleaves and deactivates factor Va and factor VIIIa, two essential components of the coagulation mechanism. This inactivation reduces the generation of additional thrombin and thereby effectively prevents continued coagulation. Reduced levels of Thrombomodulin can correlate with the pathogenesis of certain cardiovascular diseases, such as atherosclerosis and thrombosis. However, the serum levels of the truncated circulating form of Thrombomodulin are typically elevated during inflammation and in the presence of various inflammatory related diseases. Recombinant soluble Thrombomodulin is a 491 amino acid glycoprotein containing the extracellular domain of Thrombomodulin.

7215 | Human CellExp Thrombomodulin Human Recombinant DataSheet

Biomolecule/Target: Thrombomodulin

Synonyms: CD141, BDCA-3, THBD, TM

Alternates names: C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin chemotactic receptor (C5a-R), (C5aR,) (CD antigen CD88), C5AR1, C5AR, C5R1

Taglines: An endothelial cell expressed transmembrane glycoprotein

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NCBI Gene Symbol:

Gene Source:

Accession #:

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): N/A

Biological activity: Human recombinant C5a is as active as that of serum derived C5a based on enzyme release assay of myeloperoxidase. The current lot of ED = 1.36 nmolar.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: (74 amino acids)

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium phosphate, pH 7.5.

Reconstitution Instructions: Reconstitute to a concentration of 1.0 mg/ml in ice-cold HO containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C for future use. Frozen/thaw will result in some loss of activity.

Amino acid sequence: N/A