Human CellExp™ VCAM-1, Human Recombinant | 7211

VCAM is a 110 kDa cell surface integral membrane glycoprotein that belongs to the Ig-related superfamily of adhesion molecules. The primary function of VCAM-1 is the mediation of leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 may play a vital role in the development several diseases, including atherosclerosis and rheumatoid arthritis. The human VCAM-1 gene codes for a 715 amino acid transmembrane glycoprotein containing a 19 amino acid cytoplasmic domain, a 22 amino acid transmembrane domain, and a 674 amino acid extracellular domain. Recombinant human VCAM-1 is a 74.0 kDa glycoprotein comprising the extracellular domain (673 amino acid residues) of VCAM-1. Monomeric glycosylated VCAM-1 migrates at an apparent molecular weight of approximately 90.0 kDa by SDS-PAGE analysis.

7211 | Human CellExp VCAM-1 Human Recombinant DataSheet

Biomolecule/Target: VCAM1

Synonyms: CD106, INCAM-100, MGC108734, MGC99561, VCAM, VCAM1, VCAM1B, VECAM1

Alternates names: PPIB, Peptidyl prolyl isomerase B, CYPB; SCYLP; CYP-S1

Taglines: Mediates leukocyte-endothelial cell adhesion and signal transduction

NCBI Gene ID #: 5479

NCBI Gene Symbol: PPIB

Gene Source: Human

Accession #: P23284

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 220 nmoles/min/mg

Results:

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 21.2 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.0.

Reconstitution Instructions: N/A

Amino acid sequence: MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE