Human CENPF (Centromere Protein F) ELISA Kit | ELK3147

Human CENPF (Centromere Protein F) ELISA Kit | ELK3147 | Gentaur UK, US & Europe Distribution

Alternative Names: CENP-F; CENF; Hcp-1; Centromere protein F (350/400kD, Mitosin) ; AH antigen; Kinetochore protein CENPF; Mitosin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Centromere Protein F (CENPF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Centromere Protein F (CENPF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Centromere Protein F (CENPF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Centromere Protein F (CENPF) in the samples is then determined by comparing the OD of the samples to the standard curve.