Human Centromere Protein (CENP-B) ELISA Kit | KTE62684

Human Centromere Protein (CENP-B) ELISA Kit | KTE62684 | Gentaur UK, US & Europe Distribution

Application: This Human Centromere Protein (CENP-B) ELISA Kit employs a two-site sandwich ELISA to quantitate CENPB in samples. An antibody specific for CENPB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCENPB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CENPB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CENPB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Centromere Protein (CENP-B) ELISA Kit microplate
• Human Centromere Protein (CENP-B) ELISA Kit standard
• Human Centromere Protein (CENP-B) ELISA Kit detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Centromere Protein (CENP-B) ELISA Kit has high sensitivity and excellent specificity for detection of Human CENPB. No significant cross-reactivity or interference between Human CENPB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Centromere Protein B is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA.This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.

Alternative Names: CENPB; Centromere autoantigen B; centromere protein B

Search name: CENPB; Centromere autoantigen B; centromere protein B

Tag: CENPB