Human CERK (Ceramide Kinase) ELISA Kit | ELK4862

Human CERK (Ceramide Kinase) ELISA Kit | ELK4862 | Gentaur UK, US & Europe Distribution

Alternative Names: LK4; hCERK; Lipid kinase 4; Acylsphingosine kinase

Species: Human

Assay Type: Sandwich

Sensitivity: 1.14 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Metabolic pathway;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide Kinase (CERK) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ceramide Kinase (CERK) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceramide Kinase (CERK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ceramide Kinase (CERK) in the samples is then determined by comparing the OD of the samples to the standard curve.