Human CFD (Complement Factor D) ELISA Kit | ELK1128

Human CFD (Complement Factor D) ELISA Kit | ELK1128 | Gentaur UK, US & Europe Distribution

Alternative Names: CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator

Species: Human

Assay Type: Sandwich

Sensitivity: 0.257 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor D (CFD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Factor D (CFD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor D (CFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor D (CFD) in the samples is then determined by comparing the OD of the samples to the standard curve.