Human CGMP-dependent protein kinase 1 (PRKG1) ELISA Kit | KTE62344

Human CGMP-dependent protein kinase 1 (PRKG1) ELISA Kit | KTE62344 | Gentaur UK, US & Europe Distribution

Application: This Human CGMP-dependent protein kinase 1 (PRKG1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKG1 in samples. An antibody specific for PRKG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human CGMP-dependent protein kinase 1 microplate
• Human CGMP-dependent protein kinase 1 standard
• Human CGMP-dependent protein kinase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human CGMP-dependent protein kinase 1 (PRKG1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKG1. No significant cross-reactivity or interference between Human PRKG1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Two main forms of cGK have been identified: a soluble form designated type I and an intrinsic membrane-bound form designated type II. Prkg1 knockout mice showed impaired platelet responses to VWF or low doses of thrombin and prolonged bleeding time. Human platelet aggregation induced by VWF or low-dose thrombin was inhibited by PRKG1 inhibitors but enhanced by cGMP. Furthermore, a cGMP-enhancing agent, sildenafil, promoted VWF- or thrombin-induced platelet aggregation. The cGMP-stimulated platelet responses were biphasic, consisting of an initial transient stimulatory response that promoted platelet aggregation and a subsequent inhibitory response that limited the size of thrombi.

Alternative Names: PRKG1; RP11-346D6.1; CGKI; DKFZp686K042; FLJ36117; MGC71944; PGK; PKG; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha; protein kinase; cGMP-dependent; regulatory; type I; beta

Search name: PRKG1; RP11-346D6.1; CGKI; DKFZp686K042; FLJ36117; MGC71944; PGK; PKG; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha; protein kinase; cGMP-dependent; regulatory; type I; beta

Tag: PRKG1