Human CHRM2 (Cholinergic Receptor, Muscarinic 2) ELISA Kit | ELK1807

Human CHRM2 (Cholinergic Receptor, Muscarinic 2) ELISA Kit | ELK1807 | Gentaur UK, US & Europe Distribution

Alternative Names: CHR-M2; HM2; M-AChRM2; Muscarinic Acetylcholine Receptor M2; Cholinergic Receptor, Muscarinic 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.057 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Cardiovascular biology;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Muscarinic 2 (CHRM2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cholinergic Receptor, Muscarinic 2 (CHRM2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Muscarinic 2 (CHRM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Muscarinic 2 (CHRM2) in the samples is then determined by comparing the OD of the samples to the standard curve.