Human CHRNa3 (Cholinergic Receptor, Nicotinic, Alpha 3) ELISA Kit | ELK4288

Human CHRNa3 (Cholinergic Receptor, Nicotinic, Alpha 3) ELISA Kit | ELK4288 | Gentaur UK, US & Europe Distribution

Alternative Names: CHR-NA3; CHRN-A3; N-AChRA3; NAChRA3; N-AChR-A3; Neuronal Acetylcholine Receptor Alpha 3

Species: Human

Assay Type: Sandwich

Sensitivity: 0.107 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) in the samples is then determined by comparing the OD of the samples to the standard curve.