Human Chymotrypsin-like elastase family member 3B (ELA3B) ELISA Kit | KTE62403

Human Chymotrypsin-like elastase family member 3B (ELA3B) ELISA Kit | KTE62403 | Gentaur UK, US & Europe Distribution

Application: This Human Chymotrypsin-like elastase family member 3B (ELA3B) ELISA Kit employs a two-site sandwich ELISA to quantitate ELA3B in samples. An antibody specific for ELA3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyELA3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ELA3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ELA3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Chymotrypsin-like elastase family member 3B microplate
• Human Chymotrypsin-like elastase family member 3B standard
• Human Chymotrypsin-like elastase family member 3B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Chymotrypsin-like elastase family member 3B (ELA3B) ELISA Kit has high sensitivity and excellent specificity for detection of Human ELA3B. No significant cross-reactivity or interference between Human ELA3B and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays..

Alternative Names: ELA3B; RP11-26H16.3; CBPP; E1; EL-1; ELA3B; cholesterol-binding pancreatic protease; elastase 1; elastase 3B; pancreatic; fecal elastase 1; pancreatic elastase 1; pancreatic endopeptidase E; protease E; proteinas

Search name: ELA3B; RP11-26H16.3; CBPP; E1; EL-1; ELA3B; cholesterol-binding pancreatic protease; elastase 1; elastase 3B; pancreatic; fecal elastase 1; pancreatic elastase 1; pancreatic endopeptidase E; protease E; proteinas

Tag: ELA3B